Composite

Part:BBa_M1457:Design

Designed by: Eric B Anderson   Group: UtahState_BE5930_-_S11   (2012-04-24)


Opioid + transmembrane


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 380
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 380
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 380
    Illegal BglII site found at 300
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 380
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 380
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Some may need to have mutagenesis PCR reactions to remove illegal EcoR1 sites


Source

From existing basic parts

References